The application of flow cytometry for kelp meiospore isolation

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Abstract: 

The goal of this study was to evaluate flow-cytometric techniques for isolating individual kelp meiospores into 96-welled plates. Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle pressure of the commercially available, JSAN (Bay Bioscience Co. Ltd) instrument. We monitored growth and gametophyte development post-sorting for 10 months. Our data demonstrate that successful kelp meiospore isolations of up to 76% viability can be achieved with flow-cytometry. This method can save time as compared to traditional, manual isolations using pipettes and improves confidence that self-fertilized individuals will not contaminate specific crosses of resulting gametophytes. Our results highlight a new application for the flow cytometer to produce clonal kelp gametophytes with direct applications for germplasm and culture collection development.

Author(s): 
Yarish, Charles
Author(s): 
Simona Augyte
Gary H. Wikfors
Steve Pitchford
Michael Marty-Rivera
Schery Umanzor
Scott Lindell
David Bailey
Keywords: 
Chlorophyll a
Cytometry
Meiospores
Saccharina
Single cell sorting
Link / Ref: 
Research Gate
Attachment: 
PDF icon Flow cytometry - Kelp.pdf
Article Source: 
Algal Research 46
Category: 
Aquaculture methods
Processing methods
Seaweed composition